Welcome to the course website of the doctoral course in 3D reconstruction by serial sectioning of histological samples
24-26th November 2010
Course successfully completed
Course description
The course will teach practical steps in fixing histological samples, serial sectioning, taking pictures with transmission electron microscope, and 3D reconstruction. The method is an extension of studying single histological sections by sequencing them, stacking the images, and defining organelles in colour for a complete 3D image. The main instructor of the course will be professor Rustem Uzbekov from the Laboratory of Cell Biology (Electron Microscopy), University of Tours, France.
The applications cover areas such as:
- basic microbiology.
- pathogen interaction with host cells.
- morphological studies.
- teaching.
course contents
Students will get theoretical introduction to the principles, hands-on
experience with the steps in 3D reconstruction, and tools to continue
practising and working with the method at their home university. The
students will get relevant literature to read for the course and a written
assignment on applications in their own work.
Lunch and breaks are covered by the course.
Lunch and breaks are covered by the course.
The course is worth 3 AP (4.5 EAP).
- Day 1 - Theory, sectioning of samples
- Day 2 - Pictures using electron microscopy
- Day 3 - 3D reconstruction of images
course venue
Estonian University of Life Sciences, Kreutzwaldi 62, Tartu, Estonia
Images from course
Student feedback
Rustem Uzbekov is a professional a very good at knowing EM (electron microscopy), making serial sections and 3D reconstruction. I was honored to have him to teach us. Teachers could have explain simple or obvious material to the students too, like how or what are the methods that EM works.
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This course was one of the most rare ones I have taken part on here in Estonia. It was very well prepared and thus very effectively using the time-frame. The transfer of knowledge occured in an easily understandable way and the practical application gave oppotunity to "grap" the difficulties of the applied techniques. The only proposal I could give is perhaps to mention the time the program is needing to be intalled.
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The course was very interesting and showed a different way for both using EM in a different way and showing a new way to study things on a small scale. The orginasation was very good, the competence of teachers excellent; all the programs were made available before the actual course. This course exceeded my expectations.
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Good experience. Could be more effective if lasted longer but shorter days. More hands on would be good if there were more microtomes. I think people got the initial idea of what TEM techniques might be.
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I found this course really interesting, even better than I expected. The information given is useful to keep in mind at least how EM works. In addition, I've found outthat this technique could solve lots of problems related to my research, thus I am interested in going deeply into its knowledge. Concerning to the organisation of the course, I've found it perfect, congratulations.
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The course met my expectations in every sense. Just one comment: On the first day the start of the lecture could have been a little later, since it is difficult to arrive by 8 O'clock if you are not in Tartu. Otherwise everything else was excellent.
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The overall organisation of the course and the teachers was excellent. The difficulty level was appropiate & the practical hands-on approach of teaching was a very good idea. I think it was unnessecary to go to the city centre for lunch... I liked the size of the group, 20 people as planned would have been too many for everyone to try doing the things...
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Additional information
Funding options for scientific collaboration between Estonia and France
Presentation of the methods principles by Gerard Prensier
Laboratory of Cell Biology (Electron Microscopy), University of Tours, France.
Presentation on a neurological study using smilar methods